Blood samples were centrifuged at 4,500 rpm for 10 min (Dupont, Newtown, CT), and the ICG concentration was measured in the plasma from a spectrophotometer at a wavelength of 805 nm (Spectronic Milton Ray Company, Rochester, NY). 12,18,19After rapid injection of 12.5 mg ICG 5 ml, 5-ml arterial blood samples were collected every 2 min for a total of six samples. Preinfusion PV was measured for each sheep using indocyanine green (ICG) dye (Akorn Inc., Buffalo Grove, IL). The sheep were maintained in large cages with free access to food and water during 5–7 days of postoperative recovery. After emergence from anesthesia, analgesia consisted of 0.3 mg buprenorphine via intramuscular injection twice a day or as needed (Reckitt and Colman Pharmaceuticals, Richmond, VA). h −1, and the catheters were connected to continuously flushed transducers.After surgery, lactated Ringer's solution was continuously infused at 2.0 ml 17The following indwelling catheters were inserted: bilateral femoral arterial and venous catheters (Intracath Becton Dickinson, Sandy, UT) and a pulmonary arterial thermodilution cardiac output catheter (Edwards Lifesciences, Irvine, CA) through the internal jugular vein. Each sheep had a splenectomy performed under halothane anesthesia to remove the effects of splenic contraction and allow hemoglobin dilution to be used as a direct measure of PV expansion or contraction. All animals were surgically prepared in sterile operative conditions more than 5 days before the experimental protocols.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |